Description:

Course participants will be introduced to the basics of Biochemistry. The course builds on the knowledge gained in general chemistry and extends this knowledge about the chemistry of living systems. The interpretation goes through the basic building structures of biological systems (amino acids, peptides, proteins, lipids, carbohydrates, nucleic acids), biological membranes and molecular genetics to the most important metabolic processes. Particular attention is paid to the aspects necessary for understanding the methods of work in the biochemical and clinical laboratory, which are part of the follow-up chemical discipline.

The laboratories are focused on broadening the topics discussed in the lectures and their practical training, especially on the determination of biomolecules and the verification of their properties. Students should become familiar with the basic laboratory techniques of Biochemistry.

Contents:

1. Introduction to biochemistry

2. Amino-acids, peptides and proteins

3. Lipids and biomembranes

4. Carbohydrates

5. Principles of metabolism

6. Transfer of energy and information

7. Repetition

Seminar contents:

1. Laboratory regulations, safety training and introduction to biochemistry laboratory work
2. Preparation of solutions and basics of pipetting
3. TLC of amino acids and DNA isolation
4. Properties, precipitation and denaturation of proteins
5. Determination of carbohydrates
6. Properties of lipids and enzymes
7. Compensation of exercises, completing the credit tequirements

Recommended literature:

[1] LIEBERMAN, Michael a Rick E. RICER. Biochemistry, molecular biology, and genetics. 6th ed. Philadelphia, 2014.

[2] Murray, R.K., et al.: Harper´s illustrated biochemistry. Mc Graw Hill Medical, New York 2009.

[3] Nelson, D.L., Cox, M.M.: Lehninger principles of biochemistry, WH Freeman, New York 2008.

[4] Voet, D., Voet, J.G.: Biochemistry. Wiley, Hoboken 2011.

Abbreviations used:

Semester:

• W ... winter semester (usually October - February)
• S ... spring semester (usually March - June)
• W,S ... both semesters

Mode of completion of the course:

• A ... Assessment (no grade is given to this course but credits are awarded. You will receive only P (Passed) of F (Failed) and number of credits)
• GA ... Graded Assessment (a grade is awarded for this course)
• EX ... Examination (a grade is awarded for this course)
• A, EX ... Examination (the award of Assessment is a precondition for taking the Examination in the given subject, a grade is awarded for this course)

Weekly load (hours per week):

• P ... lecture
• C ... seminar
• L ... laboratory
• R ... proseminar
• S ... seminar