Description:

Aim/objectives: to introduce students to the theory of electrical phenomena at the cell, organ and organism level, to
the possibilities of measuring and using these manifestations. A sub-objective is to enable students to experimentally verify the
knowledge.
This course builds on Anatomy and Physiology I and II and requires a basic
knowledge of the structure (anatomy) and function (physiology) of the following systems (excitable tissues): nervous, musculoskeletal, circulatory
(especially the heart).
The course deals with the problems of excitable tissues (nervous,
The course deals with the physiology of nervous tissue, muscle and glandular tissue and provides knowledge of the physiology of electrical processes at different levels: cell, tissue, organ, organism.

Contents:

Lecture outline:
1. Fundamentals of electrophysiology. Structure of the cell membrane.
2. Principles of action potential propagation.
3. Genesis of the eclectic impulse
4. Special electrophysiology.
5. Special electrophysiology. Electrophysiology of the heart
6. Physical concepts of electrophysiology in terms of individual sub-issues - ?Charge transfer - the driving force of cellular function?
7. Physical concepts of electrophysiology in terms of individual sub-issues - ?And ions: transfer and storage of information?. Summary of the topics covered, preparation for the examination, answers to any questions on the topics covered, possible consultation.

Seminar contents:

1. Transepithelial electrophysiology. Demonstration -Ussing chamber with biological object. Transepithelial stresses, passive and active ion transport, ion channels, Na/K pump, pharmacology.
2. Extracellular electrophysiology of isolated nerve. Demonstration - chamber with nerve. Compound action potential. Rate of action potential propagation through nerve, Refractory period, Excitability and lability, Basic properties of action potential propagation through nerve fiber, I/t curve.
3. Electrocardiography. Demonstration - ECG recording method and basic ECG evaluation. Principles of the ECG method. Mechanism of ECG waveform formation. Components of the ECG curve. Determination of heart rate using ECG. Effect of physical activity on electrocardiographic parameters. Evaluation of pathological ECGs.

Recommended literature:

[1] SILBERNAGL, Stefan a Agamemnon DESPOPOULOS. Color atlas of physiology. 7th edition. Stuttgart: Thieme, 2015. Thieme Flexibook. ISBN 978-3-13-545007-0.
[2] PURVES, Dale. Neuroscience. 5th ed. Sunderland, Mass.: Palgrave, c2012. ISBN 978-0-87893-695-3.
[3] Principles and practice of clinical electrophysiology of vision. 2nd ed. Editor John R. HECKENLIVELY, editor Geoffrey B. ARDEN, editor Steven NUSINOWIT, editor Graham F. HOLDER, editor Michael BACH. Cambridge:MIT Press, c2006. ISBN 0-262-08346-9.

Abbreviations used:

Semester:

• W ... winter semester (usually October - February)
• S ... spring semester (usually March - June)
• W,S ... both semesters

Mode of completion of the course:

• A ... Assessment (no grade is given to this course but credits are awarded. You will receive only P (Passed) of F (Failed) and number of credits)
• GA ... Graded Assessment (a grade is awarded for this course)
• EX ... Examination (a grade is awarded for this course)
• A, EX ... Examination (the award of Assessment is a precondition for taking the Examination in the given subject, a grade is awarded for this course)

Weekly load (hours per week):

• P ... lecture
• C ... seminar
• L ... laboratory
• R ... proseminar
• S ... seminar