Description:

Knowledge of macromolecular structure is crucial for the understanding of its function. The subject is focused on the introduction to building blocks of macromolecules, overall structure and its structure:function relationship including macromolecular complexes.

Contents:

1. Chemical bonds, proteins, saccharides, nucleic acids
2. Primary, secondary, tertiary and quarternary structure of proteins
3. Protein folding
4. Structure of nucleic acids, interaction motives of protein:nucleic acids complexes
5. Basic biophysical methods, methods of structure determination of macromolecules
6. Macromolecular crystallography
7. Structure of enzymes, drug design
8. Structure of viruses and viral proteins
9. Structure and function of membranes and membrane proteins
10. Structure and function of components of immune system
11. Large macromolecular complexes (respiration, transcription, translation)

Seminar contents:

Individual work covers composition of several protocols according to the manual and independent analysis of an actual structure biology paper.

Recommended literature:

Compulsory literature:
C. Branden, J. Tooze, Introduction to Protein Structure 2nd Edition, Garland Science, New York, NY, 1999.
A. Liljas, L. Liljas, J. Piskur, G. Lindblom, P. Nissen, M. Kjeldgaard, Textbook of Structural Biology, World Scientific Publishing Co. Pte. Ltd., Singapore, 2009.

Optional literature:
B.Alberts, D. Bray, A. Johnson, J. Lewis, M. Raff, K Roberts, P. Walter, Základy buněčné biologie, Espero Publishing, s.r.o., Ústí nad Labem, 2005.
Z. Vodrážka, P. Rauch, J. Káš, Enzymologie, Vysoká škola chemicko-technologická, 1998.

Keywords:

Macromolecules, structure, enzymes, biotechnology, virus, drugs, membrane, immunity, macromolecular complexes, cell.

Abbreviations used:

Semester:

• W ... winter semester (usually October - February)
• S ... spring semester (usually March - June)
• W,S ... both semesters

Mode of completion of the course:

• A ... Assessment (no grade is given to this course but credits are awarded. You will receive only P (Passed) of F (Failed) and number of credits)
• GA ... Graded Assessment (a grade is awarded for this course)
• EX ... Examination (a grade is awarded for this course)
• A, EX ... Examination (the award of Assessment is a precondition for taking the Examination in the given subject, a grade is awarded for this course)

Weekly load (hours per week):

• P ... lecture
• C ... seminar
• L ... laboratory
• R ... proseminar
• S ... seminar