Description:

Overview of selected modern instrumental methods of research and analysis, theoretical fundamentals, instrumental technique, utilization and application.

Contents:

Overview of basic types of radiation sources and detectors used in basic techniques of analytical methods, principles of these methods, introduction to selected advanced instrumental methods of research and analysis, utilization and application.
Outline:
1. Overview of basic radiation sources and principles for X-ray, UV-VIS, IR
2. Overview of basic detectors and principles of detection
3. Basics of spectroscopy
4. Basics of vacuum technology (sources, measurements)
5. Selected analytical methods
? UV-VIS absorption spectrometry
? Atomic absorption spectrometry
? Infrared absorption spectroscopy (basic FTIR techniques)
? Absorption methods on beams of synchrotron radiation
? Raman spectroscopy
? Methods based on emission of radiation (Luminescent spectrometry (TRLFS, LIBS, etc.), X-ray photoelectron spectroscopy)
? Mössbauer spectroscopy
? Nuclear magnetic and paramagnetic resonance
? Chromatographic analytical methods (High performance liquid chromatography, Gas chromatography)
? Mass spectrometry (MS)
? Thermal analysis methods (eg differential thermal analysis, thermogravimetry, DSC, TD, EGA, ETA)
? Modern microscopy ( electron microscopy, fluorescence microscopy (SIM, STED, STORM, etc.)

Recommended literature:

Povinná literatura:
H. Gunzler et. al.: Handbook of Analytical Technichniques, Willey-VCH Verlag GmbH, Weinheim 2002.
Naito A.: Experiemntal Approaches of NMR Spectroscopy. Methodology and Application to Life Science and Materials Science. Springer 2018
Y. Engelborghs, A. J.W.G. Visser (Eds.): Fluorescence Spectroscopy and Microscopy, Humana Press, 2014

Další doporučená literatura:
J.H. Gross: Mass Spectrometry. Springer 2017.

Abbreviations used:

Semester:

• W ... winter semester (usually October - February)
• S ... spring semester (usually March - June)
• W,S ... both semesters

Mode of completion of the course:

• A ... Assessment (no grade is given to this course but credits are awarded. You will receive only P (Passed) of F (Failed) and number of credits)
• GA ... Graded Assessment (a grade is awarded for this course)
• EX ... Examination (a grade is awarded for this course)
• A, EX ... Examination (the award of Assessment is a precondition for taking the Examination in the given subject, a grade is awarded for this course)

Weekly load (hours per week):

• P ... lecture
• C ... seminar
• L ... laboratory
• R ... proseminar
• S ... seminar