Description:

In the general part of the lecture students become familiar with the preparation of enriched stable nuclides and radionuclides, nomenclature of labelled compounds, basic principles of safety and specific requirements for laboratory equipment and experimental setup for work with isotopes.
Next, laboratory operations with labelled compounds and methods of structural, isotopic and radiometric analyses are discussed, together with specificity of carrier-added and carrier-free preparations, fast and online syntheses, automated syntheses, biosyntheses.
In the systematic part of the lecture, the isotope-specific methods of the most common elements are particularly discussed, together with the applications of labelled compounds.

Contents:

1. General part
- Preparation of enriched stable nuclides and radionuclides.
- Nomenclature of labelled compounds.
- Laboratory equipment, experimental setup, safety
- Reaction apparatuses, preparations in micro- and macro- scale
- Methods of structural, isotopic and radiometric analyses
- Specificity of carrier-added and carrier-free preparations
- Fast and online syntheses, automated syntheses, biosyntheses
2. Systematic part
- Syntheses with hydrogen isotopes
- Syntheses with carbon isotopes
- Syntheses with nitrogen isotopes
- Syntheses with sulphur isotopes
- Syntheses with phosphorus and oxygen isotopes
- Syntheses with halogenides isotopes
- Syntheses with other nuclides isotopes
3. Aplications of labelled compounds

Recommended literature:

Key Literature:
1. Voges R., Heys J.R., Moenius T.: Preparation of compounds labeled with Tritium and Carbon-14. John Wiley & Sons, Ltd. 2009. ISBN: 978-0-470-51607-2.
2. Wolfe R.R., Chinkes, D.L.: Isotope tracers in metabolic research.2nd edition, John Wiley & Sons, Ltd. 2005. ISBN: 978-0-471-46209-5.
Recommended Literature:
1. Buncel E., Lee C.C. eds.: Isotopes in organic chemistry, Vol.1-3, Elsevier 1975.
2. Murray A. III., Williams D.L.: Organic syntheses with isotopes. Interscience publishers Inc. New York, 1958.

Keywords:

isotopes, synthesis

Abbreviations used:

Semester:

• W ... winter semester (usually October - February)
• S ... spring semester (usually March - June)
• W,S ... both semesters

Mode of completion of the course:

• A ... Assessment (no grade is given to this course but credits are awarded. You will receive only P (Passed) of F (Failed) and number of credits)
• GA ... Graded Assessment (a grade is awarded for this course)
• EX ... Examination (a grade is awarded for this course)
• A, EX ... Examination (the award of Assessment is a precondition for taking the Examination in the given subject, a grade is awarded for this course)

Weekly load (hours per week):

• P ... lecture
• C ... seminar
• L ... laboratory
• R ... proseminar
• S ... seminar