Description:

Students get knowledge of practical work with mikroorganisms and ezymes, including preparation of samples for irradiation. They get knowledge of basic aspects of work with radionuclide generator, preparation of labelled compounds and determination of radiochemical purity.

Seminar contents:

1. Influence of UV radiation on enzymes
2. Influence of ionizing radiation on enzymes
3. Influence of UV radiation on mikroorganisms
4. Influence of ionizing radiation on mikroorganisms
5. Radionuclide (technetium) generator
6. Labelled compounds
7. Determination of radionuclidic purity
8. Determination of radiochemical purity of compound labelled with radiocarbon

Recommended literature:

Key references:
1.Decree of the SÚJB No. 307/2002 Coll., on Radiation Protection, Annexes 1-13
2.W.D.Loveland, D.J. Morrissey, G.T. Seaborg: Modern nuclear chemistry, John
Wiley & Sons, New Jersey, 2006.
Recommended references:
1. Act No. 18/1997 Coll., on Peaceful Utilisation of Nuclear Energy and Ionising
Radiation (the Atomic Act) and on Amendments and Additions to Related Acts.
Media and Tools:
1.Irradiation facility
2.Chemical laboratory
3.Flowbox

Keywords:

Radiation methods, microorganisms, enzymes, labelled compounds, radionuclide generator

Abbreviations used:

Semester:

• W ... winter semester (usually October - February)
• S ... spring semester (usually March - June)
• W,S ... both semesters

Mode of completion of the course:

• A ... Assessment (no grade is given to this course but credits are awarded. You will receive only P (Passed) of F (Failed) and number of credits)
• GA ... Graded Assessment (a grade is awarded for this course)
• EX ... Examination (a grade is awarded for this course)
• A, EX ... Examination (the award of Assessment is a precondition for taking the Examination in the given subject, a grade is awarded for this course)

Weekly load (hours per week):

• P ... lecture
• C ... seminar
• L ... laboratory
• R ... proseminar
• S ... seminar